Identification of Regions of the Tomato γ-Glutamyl Kinase That Are Involved in Allosteric Regulation by Proline*
نویسندگان
چکیده
‡Department of Biological Sciences, Purdue University, West Lafayette IN 479071-1392, U.S.A. §Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette IN 47907 U.S.A. ¶Present Address: National Institute for Basic Biology, 38 Nishigounaka, Myo-daiji-cho, Okazaki 444-8585, Japan ||Present Address: National Center for Alternative Energy, Environment, and Innovative Technology, C. R. Trisaia, S. S. Jonica Km-419-500, Rotondella (MT), Italy †Present Address: Department of Na we added the sentenceational University, Laredo TX 78041
منابع مشابه
Identification of regions of the tomato gamma-glutamyl kinase that are involved in allosteric regulation by proline.
The first step of proline biosynthesis is catalyzed by gamma-glutamyl kinase (GK). To better understand the feedback inhibition properties of GK, we randomly mutagenized a plasmid carrying tomato tomPRO1 cDNA, which encodes proline-sensitive GK. A pool of mutagenized plasmids was transformed into an Escherichia coli GK mutant, and proline-overproducing derivatives were selected on minimal mediu...
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